HPLC法建立左金丸及类方水提液的特征指纹图谱,计算各特征峰的相对峰面积,并采用中药色谱指纹图谱相似度评价系统A版(2004 A)计算类方间的相似度;微量热法测定大肠杆菌在左金丸及类方水提液作用下的热谱曲线,得到相应的热动力学参数,如生长速率常数k,最大产热功率Pm,最高峰的出峰时间tm和总产热量Qt等,并对这些热动力学参数进行主成份分析.典型相关分析法对左金丸及类方HPLC指纹图谱中特征峰的相对峰面积与其类方作用下大肠杆菌生长代谢的主要热动力学参数相关联,研究“谱-效”相关性.结果表明,HPLC指纹图谱与生物热活性存在很好的相关性,类方中吴茱萸次碱、盐酸巴马汀、盐酸小檗碱的含量差异是导致其生物热活性不同的主要原因. The characteristic fingerprints of Zuojin Pills and similar aqueous extracts were established by HPLC, and the relative peak areas of each characteristic peak were calculated, and the similarities between similar parties were calculated using the similarity evaluation system of traditional Chinese medicine chromatographic fingerprinting (A) (2004 A); The thermograms of E. coli in Zuojin Pills and similar aqueous extracts were determined by thermal method to obtain the corresponding thermodynamic parameters, such as the growth rate constant k, the maximum heat generation power Pm, the peak peak time tm and the total Heat production Qt, etc., and the main component analysis of these thermodynamic parameters. Typical correlation analysis of the relative peak area of ​​the peaks in the HPLC fingerprint of Zuojin Pills and similar formulas and the main thermal dynamics of E. coli growth and metabolism under the effect of all kinds of side. The parameters were correlated and the “spectrum-effect” correlation was studied. The results showed that there was a good correlation between HPLC fingerprinting and bio-thermal activity. The quasi-squares of ruthenium, palmatine hydrochloride, and berberine hydrochloride were well-known. The difference in content is the main reason leading to the different biological activity.