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目的 探讨甲基对硫磷 (M160 5 )的易感性生物标志物。方法 检测M160 5作业工人 ( 71名 )及对照组 ( 5 0名 )的对氧磷酶 (PON)基因在 192位点和 5 5位点的多态性分布 ,分析各基因型与全血胆碱酯酶 (ChE)活力、免疫功能和内分泌水平的关系。结果 在全部 12 1名研究对象中 ,PON在 192位点和 5 5位点基因型均为多态性分布 ;基因型分布及等位基因频率性别之间差异无显著性 (P >0 .0 5 )。在 71位M160 5接触者中 ,PON基因192位点为谷氨酸 (Gln) /Gln的基因型者 ,其T淋巴细胞亚群中CD4、CD4/CD8分别为 4 1.3± 5 .3、1.5 6± 0 .15 ,比对照组 ( 4 9.0± 8.1、1.83± 0 .10 )和M160 5接触组精氨酸 (Arg) /Arg基因型者 ( 4 4.9± 4 .8、1.76± 0 .14 )明显降低 ,差异有显著性 (P <0 .0 5、P <0 .0 1)。PON基因 5 5位点不同基因型者M160 5的免疫毒性作用差异无显著性 (P >0 .0 5 )。结论 PON基因在 192位点Gln/Gln基因型可能是M160 5免疫毒性作用的易感性生物标志物之一。
Objective To investigate the susceptibility biomarkers of methyl parathion (M160 5). Methods The polymorphisms of PON gene at 192 sites and 5 5 sites of M160 5 workers (71) and control group (50) were detected. The Relationship between Cholesterol Esterase Activity, Immune Function and Endocrine Level. Results In all 12 subjects, PON genotypes were polymorphic at 192 loci and 55 loci; there was no significant difference in genotype distribution and allele frequency between controls (P> 0.05) 5). Of the 71 M160 5 contacts, the genotype of PON gene at position 192 was Gln / Gln, and CD4, CD4 / CD8 in T lymphocyte subsets were 41.3 ± 5, 6 ± 0.15, which was significantly higher than that of control group (9.09 ± 8.1, 1.83 ± 0.10) and M160 5 contact group (Arg / Arg genotype) (4.9 ± 4.8, 1.76 ± 0.14 ) Was significantly lower, the difference was significant (P <0.05, P <0.01). There was no significant difference in the immunotoxicity of M160 5 with different genotypes of PON gene at 5 5 loci (P> 0.05). Conclusion The Gn / Gln genotype of PON gene at 192 locus may be one of the susceptible biomarkers of M160 5 immunotoxicity.