论文部分内容阅读
本文利用人αD型干扰素基因信号多肽编码区内自身的TGATG序列,在大肠杆菌中高效表达非融合的人αD型干扰素。发现“翻译联结”(ATG-TGATG)方式起始的基因表达水平比一般ATG起始方式高约10倍;证明大肠杆菌能够识别并加工人αD型干扰素信号多肽。发现SV_(40) DNA HindⅢ B片段对人αD型干扰素基因在原核细胞中表达有增强作用。
In this paper, the non-fusion human αD-type interferon is efficiently expressed in E. coli by using its own TGATG sequence in the coding region of human αD interferon gene signal polypeptide. It was found that the gene expression level of ATG-TGATG mode was about 10 times higher than that of the general ATG initiation mode; it was confirmed that Escherichia coli can recognize and process human? D-type interferon signal polypeptide. It was found that SV_ (40) DNA HindⅢB fragment could enhance the expression of human αD interferon gene in prokaryotic cells.