物体表面的细菌总数是指从受检物品表面采样,经一定条件下培养,检出物体表面一定面积上所含的细菌总数。为了观察对同一面积上不同次数反复涂抹采样对细菌捕获率的影响,我们对驻京某部招待所、接待中心客房的茶具、卧具、毛巾以及餐厅的碗、筷等餐饮具作采样检验。现将结果报告如下。1采样方法第1次涂抹,用浸湿生理盐水的无菌棉拭,在边长5cm的规格平面上有序地涂抹一遍,用无菌剪刀将棉拭头剪入10ml生理盐水试管中;第2次涂抹,另取1支棉拭在同一物体同一块表面上,按上述方法再涂抹一遍,将棉拭头剪入另一生理盐水试管中,以两次所得菌数之和作为两次涂抹法的结果。以相同次序和方法作第3、4次采样。将试管充分摇匀后,取1ml样液接种于平皿上并倾注营养琼脂,于37℃培 The total number of bacteria on the surface of an object refers to the total number of bacteria contained in a certain area on the surface of the object after being sampled from the surface of the object to be examined under certain conditions. In order to observe the effects of repeated smear sampling on the bacterial capture rate in different times on the same area, we sampled the tableware, tableware and other dining utensils in a guest house in Beijing, the tea room in the reception center, the bedding and towels, and the restaurant. The results reported below. 1 Sampling Method The first smear, with a sterile cotton swab soaked in saline, the side of the order of 5cm on the specifications of the level of smear again with a sterile scissors cotton swab into the 10ml physiological saline test tube; 2 times smear, the other a cotton swab in the same object on the same surface, according to the above method and then smear again, the cotton swab into another physiological saline test tube to the number of bacteria twice as the sum of smear twice The result of the law. In the same order and method for the first 3, 4 sampling. After the tube was well shaken, 1ml sample solution was inoculated on the plate and nutrition agar was decanted at 37 ℃