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目的研究急性髓系白血病(AML)中WNT信号传导通路拮抗基因分泌型卷曲相关蛋白5(SFRP5)基因的甲基化状态。方法应用甲基化特异性PCR(MSP)法对99例AML患者的骨髓或外周血进行SFRP5基因启动子区甲基化状况检测,并以70例门诊普通病人的外周血作为对照。结果 99例AML患者中检出10例(10.1%)SFRP5的甲基化,70例正常对照中仅检出1例(1.4%)SFRP5的甲基化。SFRP5在AML患者中的甲基化率显著高于正常对照(P<0.05)。SFRP5基因的甲基化状态与年龄、性别无关(P>0.05),与AML的临床分型相关(P<0.05)。结论 SFRP5基因的甲基化与AML有相关性,SFRP5基因的甲基化可能是引起AML的分子机制之一。
Objective To investigate the methylation status of the gene encoding secreted Frizzled Related Protein 5 (WRP) gene of WNT signaling pathway in acute myeloid leukemia (AML). Methods Methylation-specific PCR (MSP) was used to detect the promoter methylation of SFRP5 gene in bone marrow or peripheral blood of 99 AML patients. The peripheral blood of 70 outpatients was used as control. Results Methylation of SFRP5 was detected in 10 (10.1%) of 99 AML patients and in only 1 (1.4%) of 70 normal controls. The methylation rate of SFRP5 in AML patients was significantly higher than that in normal controls (P <0.05). The methylation status of SFRP5 gene was not related to age and gender (P> 0.05), but correlated with the clinical classification of AML (P <0.05). Conclusion The methylation of SFRP5 gene is related to AML. Methylation of SFRP5 gene may be one of the molecular mechanisms that cause AML.