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分别用柔嫩艾美耳球虫早熟弱毒株、鸡胚适应株和强毒株孢子化卵囊１０００个／只，给１０日龄无球虫雏鸡免疫接种，逐日检查粪便并进行克粪便卵囊（Ｏ．Ｐ．Ｇ．）计数，结果发现早熟弱毒株和鸡胚适应株的潜隐期均有不同程度缩短，繁殖力亦有不同程度的下降。同时进行了柔嫩艾美耳球虫不同株球虫的免疫原性比较：分别用三株卵囊免疫雏鸡，免疫剂量为１０００个／只，免疫后１４ｄ各用１０００个／只强毒株卵囊攻虫，逐日进行Ｏ．Ｐ．Ｇ．计数，发现各组免疫鸡攻虫后的卵囊产量均大大下降，其中以强毒株免疫攻虫组的排出卵囊量最低，仅为未免疫组的１．２３％，早熟株和鸡胚株免疫鸡攻虫的排出卵囊量相近，分别为未免疫组的７．３５％和７．２３％。由此可见，三株柔嫩艾美尔球虫均表现出良好的免疫原性。
The Eimeria tenella precocious attenuated strains, chick embryo adaptive strains and virulent strains were sporulated oocysts 1000 / only, to 10 days old coccidia chickens immunized, daily examination of stool and grams of fecal oocysts ( O.P.G.) count, the results showed that precocious attenuated strains and chicken embryos adapt to the latent period of varying degrees of shortening, fertility also have varying degrees of decline. At the same time, the immunogenicity of different strains of Eimeria tenella were compared: the chickens were immunized with three oocysts, the immunization dose was 1000, the number of oocysts Attack worm, day by day O. P. G. Counting, it was found that the production of oocysts in the immunized chickens dropped significantly after immunized with each group. Among them, the amount of oocyst discharged by the virulent strain was the lowest, only 1.23% of the non-immunized group, The number of oocysts released by the strain of chickens immunized with chickens was similar, which were 7.35% and 7.23% of the non-immunized group respectively. Thus, all three strains of Eimeria tenella showed good immunogenicity.