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为探索银杏在水涝胁迫下的生理反应 ,进行了水涝胁迫对银杏膜脂过氧化作用及细胞保护酶活性影响的研究。结果表明 ,水涝胁迫后第 2 5d、30d ,银杏叶片中的MDA含量比对照增加了近 1倍 ,第 35d ,达到 12 1 0 4nmol/g ,比对照增加 6 4 74nmol/ g ;水涝胁迫后第 15d、2 0d、2 5d、30d、35d ,银杏叶细胞膜相对电导度分别达到 8 71%、2 0 0 5%、35 43%、4 9 6 2 %、6 6 45% ,而对照的细胞膜相对电导度为 5 70 %～ 9 32 % ;水涝胁迫下银杏叶片中MDA含量与细胞膜相对电导度呈极显著正相关 ;水涝胁迫后 15d ,银杏叶片中超氧化物歧化酶 (SOD)、过氧化物酶 (POD)、过氧化氢酶(CAT)活性升高 ,15d后酶活性降低。
In order to explore the physiological response of Ginkgo biloba under waterlogging stress, the effects of waterlogging stress on the membrane lipid peroxidation and cell protective enzyme activity were studied. The results showed that the content of MDA in Ginkgo biloba leaves increased by 1 time compared with the control at the 25th day and the 30th day after the waterlogging stress, reaching 12 1 04nmol / g at the 35th day and increased by 6 4 74nmol / g compared with the control. The waterlogging stress On the 15th, 20th, 25th, 30th and 35th day after treatment, the relative electrical conductivity of Ginkgo biloba leaf membrane reached 81 71%, 200%, 35 43%, 496 2% and 6 6 45% The relative electrical conductivity of cell membrane was from 5 70% to 9 32%. Under waterlogging stress, the content of MDA was positively correlated with the relative conductivity of cell membrane. Under the waterlogging stress, the content of superoxide dismutase (SOD) Peroxidase (POD), catalase (CAT) activity increased, after 15d enzyme activity decreased.