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目的探讨L-精氨酸(L-Arg)对乳腺癌的治疗效果及相关机制。方法制备4T1小鼠乳腺癌荷瘤模型,实验组小鼠于成瘤后连续给予灌胃L-Arg(1.5 g/kg)20 d。动态监测肿瘤组织大小和生存率,处理结束时处死小鼠,制备脾细胞悬液,经流式细胞术检测T细胞亚群和巨噬细胞比例,ELISA检测脾细胞培养上清IFN-γ和TNF-α水平,Griess反应测定上清中NO含量。结果与对照组相比,L-Arg处理可以抑制肿瘤的生长,延长小鼠生存期;同时,L-Arg处理组中脾细胞中CD4+T(26.53±4.25)%、CD8+T细胞(15.65±0.78)%和巨噬细胞(7.62±0.86)%比例明显高于PBS组[分别为(19.53±2.50)%、(8.78±1.15)%和(2.02±0.12)%],脾细胞培养上清中IFN-γ(36.56±10.50)pg/mL、TNF-α(22.05±4.93)pg/mL和NO(30.28±6.00)μm的分泌水平也明显高于PBS组[(分别为(15.47±4.96)pg/mL、(14.69±0.81)pg/mL和(6.57±2.21)μM]。结论 L-Arg可通过促进乳腺癌荷瘤小鼠的T细胞应答,发挥抗肿瘤效应。
Objective To investigate the therapeutic effect and mechanism of L-arginine on breast cancer. Methods The 4T1 mouse breast tumor model was established. The mice in experimental group were given L-Arg (1.5 g / kg) for 20 days after the tumorigenesis. Tumor size and survival rate were dynamically monitored. After the treatment, the mice were sacrificed and the spleen cell suspension was prepared. The proportion of T lymphocyte subsets and macrophages was determined by flow cytometry. The levels of IFN-γ and TNF -α levels, Griess reaction determination of NO content in the supernatant. Results Compared with the control group, L-Arg treatment could inhibit tumor growth and prolong the survival of mice. Meanwhile, the percentage of CD4 + T (26.53 ± 4.25)%, CD8 + T cells (15.65 ± 0.78% and 7.62 ± 0.86%, respectively, were significantly higher than those in PBS group [(19.53 ± 2.50)%, (8.78 ± 1.15)% and (2.02 ± 0.12)%, respectively] The levels of IFN-γ (36.56 ± 10.50) pg / mL, TNF-α (22.05 ± 4.93) pg / mL and NO (30.28 ± 6.00) μm were also significantly higher than those in PBS group [(15.47 ± 4.96, pg / mL, (14.69 ± 0.81) pg / mL and (6.57 ± 2.21) μM] .Conclusion L-Arg can exert anti-tumor effects by promoting T cell responses in breast cancer bearing mice.