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目的检测程序性死亡1(PD-1)和可溶性PD-1(sPD-1)在再生障碍性贫血(AA)患者的表达,探讨其在AA发病机制中的作用。方法采用已建立的鼠抗人PD-1分子单克隆抗体,用流式细胞术检测PD-1在AA患者(AA组)及正常人(对照组)T淋巴细胞表面的含量;用人sPD-1酶标检测sPD-1在AA患者及正常人外周血血清中的含量。结果AA组外周血T淋巴细胞上无PD-1表达,而sPD-1水平与对照组相比明显增高(P<0.01)。结论AA患者的PD-1从活化T淋巴细胞膜上脱落导致sPD-1高表达,使PD-1缺如而无法发挥负性调控作用,导致T细胞活化功能持续亢进,引起骨髓造血功能衰竭。
Objective To detect the expression of PD-1 and soluble PD-1 (sPD-1) in patients with aplastic anemia (AA) and to explore its role in the pathogenesis of AA. Methods The established mouse anti-human PD-1 monoclonal antibody was used to detect the content of PD-1 on the surface of T lymphocytes in AA patients (AA group) and normal people (control group) by flow cytometry. The content of sPD-1 in peripheral blood serum of AA patients and normal persons was detected by ELISA. Results There was no PD-1 expression in peripheral blood T lymphocytes in AA group, but the level of sPD-1 in control group was significantly higher than that in control group (P <0.01). Conclusion PD-1 shedding from the membrane of activated T lymphocytes in AA patients leads to the high expression of sPD-1, which makes PD-1 deficient and can not play a negative regulatory role, resulting in the continual activation of T cell activation and bone marrow hematopoietic failure.