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以去势雌兔作为对照,用高胆固醇饮食法复制动脉粥样硬化(AS)模型,研究了利维爱(Livial)在防治兔动脉粥样硬化过程中对雌激素受体和低密度脂蛋白受体mRNA表达的影响。应用RT-PCR方法,β-actin做内参照,对各组去势雌兔心脏、肝脏组织内雌激素受体和低密度脂蛋白受体mRNA的表达进行了检测。结果,2种受体在兔心脏和肝脏内均有表达。对照组,雌激素受体在心脏、肝脏内的相对表达量分别为0.43±0.12、0.39±0.20;低密底脂蛋白受体在心脏、肝脏内的相对表达量分别为0.27±0.05、0.86±0.12,差异显著(P<0.001)。AS模型组,雌激素受体和低密度脂蛋白受体在心脏、肝脏内的相对表达量分别为0.29±0.03、0.27±0.06和0.04±0.01、0.17±0.02,2种受体的mRNA表达均比对照组降低,差异显著(P<0.001)。利维爱组(饲喂高胆固醇饲料同时给予利维爱),雌激素受体在心脏、肝脏内的相对表达量为0.41±0.09、0.36±0.06,与模型组相比表达量显著提高(P<0.001),与对照组表达量接近;低密度脂蛋白受体在心脏、肝脏内的相对表达量为0.04±0.02、0.18±0.04,与模型组比较未见显著变化。由此认为,组织内2种受体的mRNA表达量降低可促进动脉粥样硬化的发生;利维爱通过提高组织内雌激素受体mRNA的表达可防治AS的发生与发展,对低密度脂蛋白受体的mRNA表达影响较小。
Using ovariectomized female rabbits as control, high cholesterol diet was used to replicate atherosclerosis (AS) model. The effects of Livial on the expression of estrogen receptor and low density lipoprotein Receptor mRNA expression. RT-PCR and β-actin were used as internal references to detect the expression of estrogen receptor and low density lipoprotein receptor mRNA in the heart and liver tissues of the ovariectomized female rabbits. As a result, both receptors were expressed in the heart and liver of rabbits. The relative expression levels of estrogen receptor in the heart and liver in the control group were 0.43 ± 0.12 and 0.39 ± 0.20, respectively. The relative expression levels of low density lipoprotein receptor in the heart and liver were 0.27 ± 0.05 and 0.86 ± 0.12, significant difference (P <0.001). In AS model group, the relative expression levels of estrogen receptor and low density lipoprotein receptor in the heart and liver were 0.29 ± 0.03, 0.27 ± 0.06 and 0.04 ± 0.01, 0.17 ± 0.02, respectively Lower than the control group, the difference was significant (P <0.001). Levi group (fed with high cholesterol feed and levofloxacin), the relative expression of estrogen receptor in heart and liver was 0.41 ± 0.09,0.36 ± 0.06, which was significantly higher than that in model group (P <0.001), which was similar to that of the control group. The relative expression of LDLR in the heart and liver was 0.04 ± 0.02 and 0.18 ± 0.04, which showed no significant change compared with the model group. Therefore, it is suggested that the decrease of mRNA expression of two receptors in the tissue can promote the occurrence of atherosclerosis. Levi can prevent and treat the occurrence and development of AS by increasing the expression of estrogen receptor mRNA in the tissue, Protein receptor mRNA expression less affected.