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目的探讨α1,3FUCT Ⅶ基因mRNA及Slex在非小细胞肺癌组织,癌旁正常肺组织中的表达,及其与临床病理因素的关系。方法应用RT-PCR法及免疫组织化学催化放大法(CSA)检测α1,3FUCT Ⅶ基因mRNA734.2及Slex在66例非小细胞肺癌组织,20例癌旁正常肺组织中的表达。结果α1,3FUCT Ⅶ基因mRNA在腺癌组织(0.79±0.19)强于鳞癌组织中(0.51±0.12),差异有统计学意义(P*<0.05)。Slex的表达在腺癌阳性率为86%(31/36),鳞癌为63.3%(19/30),差异有统计学意义(P*<0.05)。α1,3FUCTⅦ基因mRNA及Slex的表达与TNM分期、分化程度、淋巴结转移、组织学类型及性别有关,与年龄无关(P>0.05)。结论α1,3FUCT Ⅶ基因mRNA及Slex过表达可能与非小细胞肺癌发生及转移相关,其检测可作为判断患者肺癌转移的一项重要参考指标。
Objective To investigate the expression of α1,3FUCT Ⅶ gene mRNA and Slex in non-small cell lung cancer tissues and adjacent normal lung tissues and its relationship with clinicopathological factors. Methods The expression of α1,3FUCT Ⅶ mRNA734.2 and Slex in 66 non-small cell lung cancer tissues and 20 adjacent normal lung tissues were detected by RT-PCR and immunohistochemical catalysis (CSA). Results The mRNA expression of α1,3FUCT Ⅶ gene in adenocarcinoma (0.79 ± 0.19) was significantly higher than that in squamous cell carcinoma (0.51 ± 0.12), the difference was statistically significant (P * <0.05). The positive rate of Slex was 86% (31/36) in adenocarcinoma and 63.3% (19/30) in squamous cell carcinoma, the difference was statistically significant (P <0.05). The expression of α1,3FUCTⅦ mRNA and Slex was related to TNM stage, differentiation, lymph node metastasis, histological type and gender, but not age (P> 0.05). Conclusion The overexpression of α1,3FUCT Ⅶ gene and Slex may be related to the occurrence and metastasis of non-small cell lung cancer. The detection of α1,3FUCT Ⅶ gene and its expression may be used as an important reference index for the diagnosis of lung cancer metastasis.