细胞可透过性Cre重组酶表达、纯化及生物活性检测

来源 :生物化学与生物物理进展 | 被引量 : 0次 | 上传用户：boymaster

【摘要】

：

Cre/lox P系统由Cre位点特异重组酶和可被Cre特异性识别的lox P位点构成,该系统广泛用于条件性基因敲除和表达,以研究基因功能.为了表达和纯化一种细胞可透过性Cre重组酶(即H

【作者】

：

郭中敏徐康岳颖黄冰唐欢马芸洪迅陈系古肖东

【机构】

：

中山大学实验动物中心,中山大学第一附属医院,第三军医大学

【出处】

：

生物化学与生物物理进展

【发表日期】

：

2004年期

【关键词】

：

Cre/lox P系统细胞可透过性Cre重组酶表达纯化生物活性检测细胞和非细胞的重组系统 Cre/lox P system cell-permeabl

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论文部分内容阅读

Cre/lox P系统由Cre位点特异重组酶和可被Cre特异性识别的lox P位点构成,该系统广泛用于条件性基因敲除和表达,以研究基因功能.为了表达和纯化一种细胞可透过性Cre重组酶(即His6-NLS-Cre-MTS);经IPTG诱导,在BL21(DE3)宿主菌成功表达His6-NLS-Cre-MTS融合蛋白,通过His-Bond Ni-NTA树脂分离并纯化了该蛋白质,随后借助细胞和非细胞的重组系统成功检测了His6-NLS-Cre-MTS的生物活性.细胞可透过性Cre重组酶提供了一种快捷而高效的在细胞和在体水平进行遗传操作的新工具.

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细胞可透过性Cre重组酶表达、纯化及生物活性检测

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