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目的:对比苦石莲皮和仁中挥发性成分。方法:利用有机溶剂-水蒸气蒸馏法提取苦石莲挥发油,用GC-MS进行测定,色谱柱为HP-5MS5%Phenyl Methyl Siloxane弹性石英毛细管柱(30m×0.25mm×0.25μm),结合计算机检索技术对分离的化合物进行结构鉴定,应用色谱峰面积归一化法计算各成分的相对百分含量。结果:苦石莲皮中分离出115个化学成分,鉴定出63个化学成分,占总挥发性成分的54.7%,其中相对百分含量大于2.0%有5种;苦石莲仁中分离出102个化学成分,鉴定出51个化学成分,占总挥发性成分的50.0%,其中相对百分含量大于2.0%有7种。苦石莲皮和仁挥发性成分中有40种相同成分,其中皮中含量最高的是柠檬烯(5.31%),仁中含量最高的是己醇(12.94%)。结论:本实验首次对苦石莲皮和仁挥发性成分进行对比研究。为有效控制苦石莲药材质量及深度开发该药材提供参考数据。
Objective: To compare the volatile components of bitter pitted skin and kernels. Methods: The volatile oil was extracted by organic solvent-steam distillation and determined by GC-MS. The column was HP-5MS5%Phenyl Methyl Siloxane elastic quartz capillary column (30m×0.25mm×0.25μm), combined with computer search. The structure of the isolated compound was identified by the technique and the relative percentage of each component was calculated using the peak area normalization method. RESULTS: 115 chemical constituents were isolated from the leather of Ulva frutescens, 63 chemical constituents were identified, accounting for 54.7% of the total volatile constituents, 5 of which were relative percentages greater than 2.0%, and 102 were isolated from the hardwood lotus kernels. Ingredients, 51 chemical components were identified, accounting for 50.0% of the total volatile components, of which 7 were relative percentages greater than 2.0%. There are 40 same components in the volatile components of sarmentarius and kernels, of which the highest content is limonene (5.31%), and the highest content is hexyl alcohol (12.94%). Conclusion: This experiment is the first time to compare the volatility components of lotus skin and kernel. It provides reference data for the effective control of the quality and depth of the medicinal materials of Kuslan.