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目的观察DON毒素和氟对TGF-β2等4种骨生长发育相关指标的影响,探讨DON毒素联合元素氟致骨软骨的损伤机制。方法 28日龄Wistar大鼠40只,按照随机化方法分为空白组、氟(F)组、DON毒素组和“DON+F”组。F组和“DON+F”组饮用150 mg/L Na F溶液,空白组和DON毒素组饮用去离子水;各组的DON毒素染毒按0.2μg/g剂量每天灌胃,空白组和F组用等量蒸馏水灌胃。喂养5周后取血,ELISA法测定各细胞因子。结果血清中TGF-β2的平均含量“DON+F”组(55.47±5.07 pg/ml)>DON组(38.88±7.99 pg/ml)>F组(38.29±5.61 pg/ml)>对照组(29.20±6.12 pg/ml);血清中BMP-2的平均含量“DON+F”组(15.24±0.66 ng/ml)>F组(14.70±1.88 ng/ml)>DON组(13.94±0.70 ng/ml)>对照组(12.44±1.07 ng/ml);血清中Hyp的平均含量“DON+F”组(7.30±1.20μg/ml)F组(860.09±117.45 pg/ml)>DON组(706.52±73.72 pg/ml)>对照组(579.54±144.53pg/ml)。结论 DON毒素和F存在一定的交互作用,可导致染毒大鼠血清TGF-β2等细胞因子的变化,进而影响骨软骨的生长发育,导致病理损伤。
Objective To investigate the effects of DON toxins and fluorine on the growth and development of four bone morphogenetic proteins, such as TGF-β2, and to explore the mechanism of DON poison combined with elemental fluorine-induced osteochondral injury. Methods Forty-eight Wistar rats aged 28 days were divided into blank group, F group, DON toxin group and “DON + F” group according to the randomized method. Group F and DON + F were given 150 mg / L NaF solution, while those in blank group and DON toxin group were treated with deionized water. DON poison in each group was given intragastrically at a dosage of 0.2 μg / g, while in blank group And F group with the same amount of distilled water gavage. After 5 weeks of feeding, blood samples were taken for determination of cytokines by ELISA. Results The average content of TGF-β2 in the serum was significantly higher in the “DON + F” group (55.47 ± 5.07 pg / ml)> DON group (38.88 ± 7.99 pg / ml)> F group (38.29 ± 5.61 pg / (29.20 ± 6.12 pg / ml). The average content of BMP-2 in serum was significantly higher in DON group (15.24 ± 0.66 ng / ml) than in F group (14.70 ± 1.88 ng / ml) 0.70 ng / ml)> control group (12.44 ± 1.07 ng / ml); mean Hyp level in serum “DON + F” group (7.30 ± 1.20 μg / DON group (706.52 ± 73.72 pg / ml)> control group (579.54 ± 144.53 pg / ml). Conclusions The interaction between DON toxin and F may lead to changes of serum TGF-β2 and other cytokines in rats, which may affect the growth and development of osteochondral and lead to pathological damage.