论文部分内容阅读
目的建立骨细粒棘球蚴病动物模型,为探索骨包虫病的发生、发展规律提供试验技术平台。方法30只长爪沙鼠,于骨膜下、骨髓腔、腰椎旁直接注射接种细粒棘球蚴混悬液,建立骨包虫病动物模型。并于感染后3、6、12个月作X线及病理检查。结果长爪沙鼠骨膜下、骨髓腔、腰椎旁接种均可感染细粒棘球蚴,感染率分别为50.00%、50.00%和16.67%。接种6个月后X片示骨膜反应;12个月放大1.64倍X光片可明确骨骼破坏情况。病理检查示,骨包虫没有包虫膜,囊肿具有外生性特征。结论成功建立骨细粒棘球蚴病长爪沙鼠动物模型,于长爪沙鼠的骨髓腔和骨膜下接种感染率较高,死亡率低;腰椎旁接种感染率偏低,且死亡率高。
Objective To establish an animal model of Echinococcus granulosus, and provide experimental technology platform for exploring the occurrence and development of. Methods Thirty gerbils (Meriones unguiculatus) were injected subcutaneously into the subperiosteum, marrow cavity and lumbar vertebra. And 3,6,12 months after infection for X-ray and pathological examination. Results Echinococcus granulosus was infected by subperiosteal, intramedullary, lumbar and paravertebral parasitoid, respectively. The infection rates were 50.00%, 50.00% and 16.67% respectively. Six months after inoculation X showed periosteal reaction; 12 months to enlarge 1.64 times X-ray can be clear bone destruction. Pathological examination showed that there was no hydatid cyst in hydatid cyst, and cyst had extrinsic characteristics. Conclusion The animal model of gerbil was established successfully. The infection rate of marrow cavity and subperiosteum in Meriones unguiculatus was high and the mortality rate was low. The infection rate of lumbar parainfluence was low and the mortality rate was high .