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选择16例血清HBsAg阳性患者为实验组,19例血清HBsAg阴性患者为对照组,每一患者同时采取血清和骨髓涂片,用免疫细胞化学方法(PAP)检测骨髓涂片细胞中的HBsAg。结果,实验组3例骨髓细胞HBsAg阳性者,其血清中HBsAg滴度都很高,而且HBeAg均呈阳性。而在抗HBe阳性或HBeAg/HBeAb阴性者中均无骨髓细胞HBsAg阳性者。在5例血清HBV-DNA多聚酶阳性者中,骨髓细胞中HBsAg阳性者2例;6例多聚酶阴性者中,骨髓细胞中HBsAg阳性者仅1例。 本研究结果证明,HBV可在肝外组织细胞中测出,骨髓细胞HBsAg阳性的出现有集中于HBV高水平复制感染者中的倾向,同时更常见于HBV感染的较早时期。
Sixteen serum HBsAg positive patients were selected as experimental group and 19 serum HBsAg negative patients as control group. Serum and bone marrow smears were taken simultaneously in each group. HBsAg in bone marrow smear cells was detected by immunocytochemical method (PAP). Results, 3 HBsAg-positive bone marrow cells in the experimental group, the serum HBsAg titers were high, and HBeAg were positive. However, none of the HBsAg-positive patients had anti-HBe positive or HBeAg / HBeAb negative. In 5 cases of serum HBV-DNA polymerase positive, 2 cases of HBsAg positive bone marrow cells; 6 cases of polymerase negative, bone marrow cells HBsAg positive only 1 case. The results of this study demonstrate that HBV can be detected in extrahepatic tissues and cells. HBsAg positive in bone marrow cells tends to be concentrated in patients with high levels of HBV replication, and is more common in earlier stages of HBV infection.