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为了查明内蒙古中部地区苜蓿根腐病的主要致病菌,按照柯赫氏法则对呼和浩特地区感染苜蓿根腐病的317个样品进行病原物分离鉴定。结果表明:呼和浩特地区的苜蓿根腐病是由多种病原菌混合侵染所致,从发病部位分离到的菌株以镰孢霉(Fusarium spp.)为优势病原,经致病性测定和接种试验证明,茄病镰刀菌(F. solani)致病性最强,接种后发病率达90.0%,其次是尖孢镰刀菌(F. oxysporum)和黄色镰刀菌(F. culmorum),发病率分别为61.7%和81.7%。茄病镰刀菌是苜蓿根腐病的主要病原菌,与黄色镰刀菌或尖孢镰刀菌复合侵染后,发病率高于黄色镰刀菌或尖孢镰刀菌单独接种的发病率。
In order to identify the main pathogens of root rot in alfalfa in the middle part of Inner Mongolia, 317 samples of root rot of alfalfa in Hohhot were isolated and identified according to Koch’s rule. The results showed that the root rot of alfalfa in Hohhot was caused by the mixed infection of many pathogens. Fusarium spp. Was the dominant pathogen isolated from the diseased sites, and the pathogenicity and inoculation tests proved F. solani had the strongest pathogenicity with the incidence of 90.0% after inoculation, followed by F. oxysporum and F. culmorum with the incidence of 61.7 % And 81.7%. Fusarium solani is the main pathogen of alfalfa root rot, and the incidence of Fusarium oxysporum or Fusarium oxysporum was higher than that of Fusarium oxysporum or Fusarium oxysporum alone.