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【目的】研究复方磺胺甲噁唑在鲫鱼体内的药物代谢动力学(以下简称药动学)特征,为制定其合理的用药方案提供理论依据。【方法】对体质量为(68.18±14.68)g/尾的鲫鱼以500mg/kg的剂量单次口灌复方磺胺甲噁唑粉(100g含磺胺甲噁唑(SMZ)8.33g、甲氧苄啶(TMP)1.67g)混悬液,并分别于给药后0.25,0.5,0.75,1,1.5,2,4,6,8,12,24,36,48,72h采集鲫鱼血浆、肌肉、肝和肾4种组织,采用高效液相色谱-紫外检测法(HPLC-UV)对样品进行检测,通过3p97软件分析药动学参数。【结果】甲氧苄啶在鲫鱼血浆、肌肉和肾中的药动学特征符合一级吸收二室开放模型,在肝中的药动学特征符合一级吸收一室开放模型;磺胺甲噁唑在鲫鱼血浆、肌肉、肝和肾中的药动学特征符合一级吸收二室开放模型。【结论】药物的2种主要成分在健康鲫鱼体内吸收较完全,分布广泛,甲氧苄啶较磺胺甲噁唑吸收快、消除慢。
【Objective】 To investigate the pharmacokinetics of compound sulfamethoxazole in crucian carp (hereinafter referred to as pharmacokinetics) and provide a theoretical basis for formulating its rational drug regimen. 【Method】 Crude carp (68.18 ± 14.68) g / tail was infused with 500 mg / kg of compound sulfamethoxazole powder (100 g SMZ 8.33 g), trimethoprim (TMP) 1.67g) were added to the crucian carp, and the carp plasma, muscle and liver were collected at 0.25, 0.5, 0.75, 1, 1.5, 2, 4, 6, 8, 12, 24, And kidney four kinds of tissues, the samples were detected by HPLC-UV and the pharmacokinetic parameters were analyzed by 3p97 software. [Results] The pharmacokinetics of trimethoprim in the plasma, muscle and kidney of Carassius auratus accorded with the first-order absorption two-compartment open model and the pharmacokinetic characteristics of the trimethoprim in the liver accorded with the first-order absorption one-compartment open model. The sulfamethoxazole Pharmacokinetic characteristics in the plasma, muscle, liver and kidney of the carp conform to the primary absorption two compartment open model. 【Conclusion】 The two main components of the drug are relatively intact and widely distributed in healthy crucian carp. Trimethoprim absorbed faster than sulfamethoxazole and eliminated slowly.