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目的:探讨N-乙酰半胱氨酸(NAC)对博莱霉素(BLM)诱导的肺纤维化大鼠Bcl-2/Bax的影响及其作用机理。方法:通过气管内注入BLM复制大鼠肺纤维化模型,防治组于造模7 d前、治疗组于造模7 d后分别开始给予NAC进行治疗。于造模后第7、14、28天每组处死大鼠5只,取肺组织,通过透射电镜、免疫组织化学方法观察肺纤维化的变化情况以及Bcl-2/Bax的表达情况,以明确NAC对肺纤维化的疗效及其可能的作用机制。结果:与模型组比较,防治组、治疗组的肺泡炎及肺纤维化程度均明显减轻(P<0.05),且Bcl-2的减少和Bax的增加受到了抑制(P<0.05),防治组更明显。结论:通过抑制Bcl-2的降低和Bax的增加,NAC有效地抑制了BLM诱导的肺纤维化,且7 d前给药效果较7 d后显著。
Objective: To investigate the effect of NAC on Bcl-2 / Bax in bleomycin (BLM) -induced pulmonary fibrosis in rats and its mechanism. Methods: The model of pulmonary fibrosis was induced by intratracheal instillation of BLM in rats. The control group was given 7 days before model making. The treatment group was given NAC for 7 days after model making. On the 7th, 14th and 28th day after operation, 5 rats in each group were sacrificed and the lung tissue was taken out. The changes of pulmonary fibrosis and the expression of Bcl-2 / Bax were observed by transmission electron microscopy and immunohistochemistry to confirm Therapeutic effect of NAC on pulmonary fibrosis and its possible mechanism. Results: Compared with model group, the degree of alveolitis and pulmonary fibrosis in prevention group and treatment group were significantly reduced (P <0.05), and the decrease of Bcl-2 and the increase of Bax were inhibited (P <0.05) More obvious. CONCLUSION: NAC effectively inhibits BLM-induced pulmonary fibrosis by inhibiting the decrease of Bcl-2 and the increase of Bax, and the effect of 7-d administration is better than that of 7-d administration.