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[目的]了解黄芩Scutellaria baicalensis Georgi种子萌发过程中的初生与次生代谢规律。[方法]超氧化物岐化酶(SOD)活性测定采用核黄素-NBT法,过氧化物酶(POD)活性测定采用愈创木酚比色法,过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、苯丙氨酸解氨酶和肉桂酸-4-羟化酶活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。[结果]在黄芩种子萌发的前5天,可溶性糖的含量不断降低,当子叶形成时有所增高。苯丙氨酸解氨酶、肉桂酸-4-羟化酶和查耳酮合成酶均随着萌发的进行而增加,其次生代谢物质也呈现出相同的变化趋势。并且次生代谢物质与关键酶的活性呈现正相关。[结论]在种子萌发的过程中次生代谢物质的形成与关键酶呈现显著性的相关,所以我们要想获得高质量的药材可以通过采取措施提高关键酶的活性。
[Objective] To understand the primary and secondary metabolism of Scutellaria baicalensis Georgi seeds during germination. [Method] The activity of superoxide dismutase (SOD) was determined by using riboflavin - NBT method and peroxidase (POD) activity assay using guaiacol colorimetric method, catalase (CAT), ascorbic acid Oxidase (APX), phenylalanine ammonia-lyase and cinnamic acid-4-hydroxylase activity were measured by UV spectrophotometry. Secondary metabolites were determined by high performance liquid chromatography. [Result] The soluble sugar content of Scutellariae Scutellariae decreased continuously in the first 5 days and increased when the cotyledons were formed. Phenylalanine ammonia-lyase, cinnamic acid-4-hydroxylase and chalcone synthase increased with germination, and their secondary metabolites showed the same trend. And secondary metabolites and key enzyme activity showed a positive correlation. [Conclusion] The formation of secondary metabolites was significantly correlated with the key enzymes in the process of seed germination. Therefore, if we want to obtain high quality medicinal materials, we can improve the activity of key enzymes.