毛细胞白血病经常与 5q1 3.3断裂位点相关联 ,该断裂位点区域及位于这一区域的重要基因有待研究 .我们探索了 DNA纤维荧光原位杂交方法 (即 DNA纤维 FISH)检测该断裂位点的可行性 .实验选用含有断裂位点区域的两个基因组克隆及位于断裂位点两侧的两个cos质粒探针与带有结构性倒位 (5) (p1 3.1 q1 3.3)的线性 DNA共杂交 (DNA来自 HCL患者的细胞系 ) .实验证明该断裂位点将探针信号一分为二 .根据这些结果描绘出断裂位点区域图 .研究表明 ,DNA纤维 FISH方法是绘制高精度物理图谱和检出遗传重排的一种有效的研究手段 Hairy cell leukemia is often associated with the 5q1 3.3 cleavage site, and the cleavage site region and important genes located in this region are to be studied.We have explored the DNA fiber fluorescence in situ hybridization (ie, DNA FISH) to detect this cleavage site The feasibility of using two genomic clones containing the region of the cleavage site and two cos plasmid probes flanking the cleavage site and linear DNA with structural inversion (5) (p1 3.1 q1 3.3) Hybridization (DNA from HCL patient cell lines) .It has been proved by the experiment that the site of the probe splits the probe signal into two parts.According to these results, a map of the site of the fracture site is drawn.Research shows that the DNA FISH method is to draw a high-precision physical map And to detect genetic rearrangement of an effective research tool