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我们首次用分子生物学方法（PCR）对布病暴发流行的疫区-陕西省绥德县义合乡和满堂川乡54例布病病人和36例对照进行了研究，发现以SAT1：100＋＋为诊断标准诊断的布病病人中，PCR的阳性率为75％，而生活环境相同的以相同的标准判定的对照人群PCR的阳性率是56.7％，二者差异显著，P＜0．05。两组人群RBPT的阳性率分别是96.3％和30．6％。同时，我们还检查了免疫组8人，免疫时间从一个月到32年不等，PCR和RBPT的阳性率分别是100％和87.5％。对急性、亚急性和慢性期布病病人PCR的阳性率进行统计学处理，结果P＞0.10，差异不显著。这表明：以查抗体为主的血清学结果与感染或免疫的时间长短有关，而检查靶DNA的PCR方法则不受感染病期或免疫时间的影响。
For the first time, we used molecular biology method (PCR) to study 54 cases of brucellosis patients and 36 controls in Yihe Township and Manchuanchuan Township, Suide County, Shaanxi Province. The results showed that SAT1: 100 ++ The positive rate of PCR was 75% in the brucellosis patients diagnosed according to the diagnostic criteria. The positive rate of PCR in the control group with the same living standard determined by the same standard was 56.7%, the difference was significant (P <0.05). The positive rates of RBPT in the two groups were 96.3% and 30.6% respectively. At the same time, we also examined the immune group of eight people, immunization time ranging from one month to 32 years, PCR and RBPT positive rates were 100% and 87.5%. The positive rate of PCR in patients with acute, subacute and chronic sarcoidosis was statistically analyzed. The results showed that P> 0.10, the difference was not significant. This indicates that the serological results mainly based on antibodies are related to the length of infection or immunization, and the PCR methods for detecting target DNA are not affected by the infection or immunization time.