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目的 探索研究非霍奇金淋巴瘤中 p5 3mRNA的表达与突变型p5 3蛋白和临床病理的相关性。 方法 采用原位杂交和ABC法。结果 在 4 8例各型非霍奇金淋巴瘤中 p5 3mRNA有不同程度的表达 ,检出率为5 8.3 % ,突变型 p5 3蛋白染色阳性率 4 1.7% ;在突变型p5 3蛋白阳性组中 p5 3mRNA的检出率为 80 .0 % ,显著高于突变型 p5 3蛋白阴性组 ( 4 2 .8% ) (P <0 .0 5 )。 结论 在非霍奇金淋巴瘤中存在 p5 3基因突变 ,产生了突变型p5 3mRNA ,提示后者的表达增强可能是导致突变型 p5 3蛋白表达增强的主要原因之一。
Objective To explore the correlation between the expression of p53 mRNA and the mutant p53 protein and clinicopathology in non-Hodgkin’s lymphoma. Methods In situ hybridization and ABC method were used. Results The expression of p53 mRNA was detected in 48 cases of various types of non-Hodgkin’s lymphoma. The detection rate was 5 8.3%. The positive rate of mutant p53 protein was 41.7%. The positive group of mutant p53 was positive. The detection rate of p53 mRNA was 80.0 %, which was significantly higher than that of mutant p53 protein negative group ( 42.8%) (P < 0.05). Conclusion There is a p53 gene mutation in non-Hodgkin’s lymphoma and a mutant p53 mRNA is produced, suggesting that the latter’s enhanced expression may be one of the major causes of the increased expression of the mutant p53 protein.