利用已糖激酶（Ｈｅｘｏｋｉｎａｓｅ 简称ＨＫ）、６磷酸葡萄糖脱氢酶（Ｇｌｕｃｏｓｅ６ｐｈｏｓｐｈａｔｅ ｄｅｈｙｄｒｏｇｅｎａｓｅ 简称（Ｇ６ＰＤＨ）） 偶联而催化葡萄糖反应的原理，通过测定还原型烟酰胺腺嘌呤二核苷酸磷酸（Ｎｉｃｏｔｉｎａｍ ｉｄｅ ａｄｅｎｉｎｅ ｄｉｎｕｃｌｅｏｔｉｄｅ ｐｈｏｓｐｈａｔｅ ｒｅｄｕｃｅｄ ｆｏｒｍ 简称ＮＡＤＰＨ）的吸光度变化率得出其酶促反应速度，采用标准曲线法测定了葡萄糖的含量。检出限为７×１０－ ７ｍ ｏｌ／Ｌ。 By using the principle of hexokinase (HK) and glucose6phosphate dehydrogenase (G6PDH) coupling to catalyze the glucose reaction, the reducing nicotinamide adenine The rate of change of absorbance of Nicotinamide adenine dinucleotide phosphate reduced form (NADPH) was calculated as the rate of enzymatic reaction. The content of glucose was determined by the standard curve method. The detection limit was 7 × 10-7mol / L.