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目的:从噬菌体随机七肽库中筛选能与PreS1抗原特异性结合的多肽。方法:利用噬菌体展示技术,以PreS1抗原为靶分子,从随机七肽库中进行生物亲和筛选,ELISA鉴定阳性克隆。结果:对肽库进行3轮筛选后,通过ELISA和竞争抑制ELISA,获得了特异性的结合肽,测序结果显示噬菌体上的短肽有共同序列。结论:利用噬菌体展示技术成功筛选到了PreS1抗原的结合肽,为乙肝的治疗和诊断探索新的途径。
OBJECTIVE: To screen the phage random peptide library for specific binding to PreS1 antigen. Methods: Using phage display technology, using PreS1 antigen as target molecule, bioaffinity screening was carried out from random heptapeptide library, and positive clones were identified by ELISA. RESULTS: After three rounds of screening of the peptide library, specific binding peptides were obtained by ELISA and competitive inhibition ELISA. The sequencing results showed that the short peptides on the phage shared a common sequence. Conclusion: We successfully screened the binding peptide of PreS1 antigen by phage display technology and explored new ways for the treatment and diagnosis of hepatitis B virus.