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目的 了解江苏省不同人群中艾滋病病毒 (HIV )各亚型毒株的流行情况和传播规律。方法 收集HIV感染者及患者的流行病学资料 ;无菌采集HIV感染者或艾滋病 (AIDS)患者抗凝全血标本 5ml,提取前病毒DNA ,用巢式聚合酶链反应 (nested PCR)扩增膜蛋白基因的C2～V3区 ,进行序列测定 ,鉴定病毒亚型。用威斯康星GCG软件进行共享序列、基因离散率的计算和毒株的聚类分析。结果 截止 2 0 0 1年底 ,江苏省已发现HIV 1中的A、B、C、D、E五种亚型和一个B亚型变种(B’)流行 ,C(占 40 .48% )和B’(3 8.10 % )亚型为主要流行株 ;静脉吸毒感染人群中 86.67%为C亚型 ,采供血和受血感染人群中 91.67%为B’亚型 ,在性途径传播人群中所有六种亚型均有 ,且分布较为均一。结论 有偿献血人群中B’亚型毒株由邻省传入 ,吸毒人群中C亚型毒株的传入主要与新疆籍流动人口的介入有关 ;多种亚型的并存说明江苏省存在着适宜AIDS流行的各种危险因素 ;提示今后在药物治疗、疫苗研制以及其他防制工作上将面临更大的困难
Objective To understand the prevalence and transmission of HIV subtypes among different populations in Jiangsu Province. Methods Epidemiological data of HIV infected patients and patients were collected. Aseptically collected 5 ml of anticoagulated whole blood samples from HIV infected persons or AIDS patients were collected and the proviral DNA was extracted and amplified by nested PCR Membrane protein gene C2 ~ V3 region, sequence determination, identification of virus subtypes. The sequence was shared by the Wisconsin GCG software, the calculation of the gene dispersion rate and the cluster analysis of the strains. Results As of the end of 2001, five subtypes A, B, C, D and E and one subtype B (HIV) of HIV 1 were found in Jiangsu Province, with C (40.48%) and B (3 8.10%) subtype was the main epidemic strain; 86.67% of the intravenous drug users were subtype C, 91.67% of the patients receiving blood supply and blood infection were B ’subtype, and all six There are subtypes, and the distribution is more uniform. Conclusion The B subtype strains in paid blood donation groups are introduced from neighboring provinces. The introduction of subtype C subtype among drug users is mainly related to the intervention of floating population in Xinjiang. The coexistence of multiple subtypes indicates that there are suitable AIDS epidemic of various risk factors; suggesting that future drug treatment, vaccine development and other prevention work will face greater difficulties