目的　构建人端粒酶蛋白催化亚单位 (hTRT)正、反义真核表达载体。方法　采用分子克隆技术将hTRTcDNA正向及反向克隆到真核表达载体pcl neo中。结果　NotⅠ酶切后 ,正义重组基因为 8.8kb一条带 ,反义重组基因为 3 .4kb和 5 .4kb二条带 ,与理论计算相符。结论　成功构建了hTRT正反义真核表达载体 Objective To construct a positive and antisense eukaryotic expression vector of human telomerase catalytic subunit (hTRT). Methods The hTRT cDNA was cloned into eukaryotic expression vector pcl neo by reverse transcription using the molecular cloning technique. Results After digestion with Not Ⅰ, the sense recombination gene was 8.8 kb in one band and the antisense recombinant gene was 3.4 kb in size and 5.4 kb in two bands, which was consistent with the theoretical calculation. Conclusion The hTRT sense and antisense eukaryotic expression vector was successfully constructed