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以已经克隆到亚麻木质素合成关键酶咖啡酸-O-甲基转移酶基因(COMT)全长cDNA序列为基础,通过扩增RNAi顺式及反式片段(均为249 bp),先连接到中间载体pBSK,再连接表达载体pCAMBIA-1301-multi,构建成COMT基因的RNAi表达载体,通过农杆菌介导转化亚麻,获得转基因植株,通过GUS染色和PCR检测,表明干扰载体已经转入亚麻中。这为推进亚麻纤维品质改良的分子育种提供了基础。
Based on the full-length cDNA sequence of caffeic acid-O-methyltransferase gene (COMT), a key enzyme in the synthesis of flax lignin, the RNAi cis- and trans-fragments (both 249 bp) The intermediate vector pBSK was ligated with the expression vector pCAMBIA-1301-multi to construct the RNAi expression vector of COMT gene. Agrobacterium tumefaciens-mediated transformation of flax was used to obtain transgenic plants. The GUS staining and PCR showed that the interfering vector had been transferred into flax . This provides the basis for promoting molecular breeding of improved linen fiber quality.