论文部分内容阅读
目的:目前HBV感染动物模型各有局限,无法全面研究HBV。拟建立人HBV血清感染Babl/c乳鼠的动物模型,以便于研究HBV感染与乳鼠免疫力低下的相关性。方法:将20只Babl/c乳鼠随机分为实验组、对照组、PBS组及空白组,通过高压水动力尾静脉注射法将人HBV血清、正常人血清、PBS注入各组乳鼠体内,记录接种后乳鼠体温及体质量。于接种后第7、15、30 d采集血清标本,应用ELISA检测HBs Ag、HBe Ag的表达情况,实时荧光定量PCR检测HBV DNA浓度。结果:接种后各组小鼠体温及体质量均无明显变化。实验组中共4只乳鼠可检测到HBs Ag为阳性且维持时间长达30 d,但HBe Ag均为阴性,HBV DNA浓度均未达500 IU/ml;余下各组检测HBs Ag、HBe Ag均为阴性。结论:通过高压水动力尾静脉注射法接种人HBV血清,可成功使Babl/c乳鼠感染HBV。本实验证实乳鼠免疫力低下,HBV血清进入体内未能有效清除,对HBV存在易感性,为建立HBV感染动物模型提供实验依据,可用于研究HBV对免疫状态的影响。
OBJECTIVE: The present animal models of HBV infection have their limitations and are unable to fully study HBV. To establish an animal model of HBV infection in Babl / c suckling mice, in order to study the correlation between HBV infection and low immunity of the suckling mice. Methods: 20 Babl / c suckling mice were randomly divided into experimental group, control group, PBS group and blank group. Human HBV serum, normal human serum and PBS were injected into each group of mice by high pressure hydrodynamic tail vein injection. Record the temperature and weight of the suckling mice after inoculation. Serum samples were collected on the 7th, 15th and 30th day after inoculation. The expression of HBs Ag and HBe Ag was detected by ELISA and the HBV DNA concentration by real-time fluorescence quantitative PCR. Results: There was no significant change in body temperature and body weight of mice in each group after inoculation. In the experimental group, a total of 4 suckling mice could detect HBsAg positive for 30 days, but HBeAg was negative, HBV DNA concentrations were not reached 500 IU / ml; the remaining groups were detected HBsAg, HBeAg Negative. Conclusion: Babl / c suckling mice can be successfully infected with HBV by high-pressure hydrodynamic tail vein injection. This experiment confirmed that low immune immunity in neonatal rats, HBV serum into the body failed to effectively remove the presence of HBV susceptibility, to establish experimental evidence of animal models of HBV infection can be used to study the impact of HBV on immune status.