目的 研究川东獐牙菜药材的HPLC指纹图谱。方法 色谱柱为大连依利特公司HYPERSIL BDS C_(18)(4.6mm×200mm,5μm)。流动相为乙腈-磷酸二氢钠(0.03 mol·L~(-1),pH=3.0),梯度洗脱(ACN％:0 min10％,15 min 20％,30 min 35％,50 min 55％),柱温为25℃,流速为1.0 mL·min~(-1),检测波长为250 nm。结果 初步建立了川东獐牙菜药材的HPLC指纹图谱,标定了14个共有指纹峰、并利用对照品对7个共有指纹峰进行了标记、结论 利用川东獐牙菜HPLC指纹图谱可以较为全面地反映川东獐牙菜药材的内在质量,有助于该药材的标准化种植。 Objective To study the HPLC fingerprint of Swertia chuanxiong. Method The column was HYPERSIL BDS C_(18)(4.6mm×200mm, 5μm). The mobile phase was acetonitrile-sodium dihydrogen phosphate (0.03 mol·L -1), pH=3.0, gradient elution (ACN%: 0 min 10%, 15 min 20%, 30 min 35%, 50 min 55% The column temperature was 25°C, the flow rate was 1.0 mL·min -1, and the detection wavelength was 250 nm. Results The HPLC fingerprint of Swertia chuanxiong was preliminarily established. Fourteen common fingerprint peaks were calibrated, and seven common fingerprint peaks were labeled with reference substance. Conclusions HPLC fingerprints of Swertia argentinus can be more comprehensive. It reflects the intrinsic quality of Chuandong Swertia medicine and helps to standardize cultivation of the medicinal material.