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目的 探讨在体外受精 胚胎移植 (IVF ET)周期中血清及卵泡液中血管内皮生长因子(VEGF)水平的变化 ,及其在预测卵巢过度刺激综合征 (OHSS)中的作用。方法 收集 42例行IVF ET患者的血清和卵泡液标本 ,采用定量酶联免疫方法检测血清、卵泡液中VEGF水平 ,按是否发生OHSS分为OHSS组 (10例 )及对照组 (32例 ) ,对两组临床资料、性激素和VEGF水平进行回顾性相关分析。结果 OHSS组血清VEGF水平在月经周期的各个时期均高于对照组 ,但差异无显著性 (P >0 0 5 )。OHSS组卵泡液VEGF水平为 (1487 7± 36 5 8)ng/L ,较对照组的 (10 2 5 8± 474 7)ng/L明显升高 ;取卵时卵泡数目增多 ,OHSS组为 (10 0± 5 9)个 ,对照组为 (6 1± 2 3)个 ;基础黄体生成素 (LH) ,人绒毛膜促性腺激素 (hCG)、注射日雌二醇 (E2 )水平均比对照组高 ,差异有显著性 (P <0 0 5 )。结论 OHSS组卵泡液VEGF水平明显高于对照组 ,提示VEGF可能参与了OHSS的发病 ;卵泡液VEGF水平可以作为预测OHSS发生的检测指标。
Objective To investigate the changes of vascular endothelial growth factor (VEGF) in serum and follicular fluid during IVF ET and its role in the prediction of ovarian hyperstimulation syndrome (OHSS). Methods Serum and follicular fluid samples from 42 patients with IVF ET were collected. The levels of VEGF in serum and follicular fluid were detected by quantitative enzyme-linked immunosorbent assay. OHSS was divided into OHSS group (n = 10) and control group (n = 32) The clinical data, sex hormone and VEGF levels were retrospectively analyzed. Results The level of serum VEGF in OHSS group was higher than that in control group at all stages of menstrual cycle, but the difference was not significant (P> 0.05). The level of VEGF in follicular fluid of OHSS group was (1487 7 ± 36 5 8) ng / L, which was significantly higher than that of the control group (10 2 58 ± 474 7) ng / L. The number of follicles in the OHSS group was ( 10 0 ± 5 9), and the control group was (6 1 ± 2 3). The levels of basal LH, hCG and E2 were significantly higher than those of the control Group height, the difference was significant (P <0 05). Conclusion The level of VEGF in follicular fluid in OHSS group is significantly higher than that in control group, suggesting that VEGF may be involved in the pathogenesis of OHSS. The level of VEGF in follicular fluid can be used as a predictor of OHSS.