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目的探讨理想糖尿病性勃起功能障碍(ED)大鼠模型的制备方法。方法90只SD大鼠,随机分为Ⅰ、Ⅱ、Ⅲ组,每组再分为正常组(CN)和糖尿病组(DM),每组各15只,糖尿病组腹腔注射链脲佐菌素(STZ,65 mg/kg)诱导糖尿病大鼠模型,成模8、12及16周注射阿朴吗啡(APO,80μg/kg)观察大鼠阴茎勃起情况,制备糖尿病性ED大鼠模型。结果DM组在8、12、16周注射阿朴吗啡后勃起次数分别为1.0±0.0、1.0±0.0、1.0±0.0,勃起率分别为33.3%、21.4%、14.3%,ED发生率分别为66.7%、78.6%、85.7%,CN组勃起次数分别为2.2±0.8、2.3±0.8、2.0±0.7,勃起率均为100%,ED发生率为0。组间差异有统计学意义(P<0.01)。结论采用STZ 65 mg/kg腹腔注射建立糖尿病大鼠模型方法安全有效,颈部皮下注射APO 80μg/kg筛选糖尿病ED大鼠可行可靠。
Objective To explore the preparation method of ideal diabetic erectile dysfunction (ED) rat model. Methods Ninety Sprague-Dawley rats were randomly divided into four groups: group Ⅰ, group Ⅱ, and group Ⅲ. Each group was divided into normal group (CN) and diabetic group (DM), 15 in each group. Diabetic rats were given intraperitoneal streptozotocin STZ, 65 mg / kg). The morphological changes of penile erection were observed at 8, 12 and 16 weeks after injection of apomorphine (APO, 80μg / kg). Results The number of erectile events in the DM group after injection of apomorphine at 8, 12 and 16 weeks were 1.0 ± 0.0, 1.0 ± 0.0 and 1.0 ± 0.0 respectively. The erectile rates were 33.3%, 21.4% and 14.3% respectively. The incidences of ED were 66.7 %, 78.6% and 85.7% respectively. The number of erection in CN group was 2.2 ± 0.8, 2.3 ± 0.8 and 2.0 ± 0.7 respectively. The erectile rate was 100% and the incidence of ED was 0. There was significant difference between groups (P <0.01). Conclusion STZ 65 mg / kg intraperitoneal injection of diabetic rat model is safe and effective, subcutaneous injection of APO 80μg / kg into the neck of diabetic rats is feasible and reliable.