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目的研究BisⅧ对Jurkat T细胞活化、增殖、周期和凋亡的影响,并对其抗肿瘤机制进行初步探讨。方法以Jurkat T细胞为模型;以双色荧光抗体染色技术结合流式细胞术检测BisⅧ对T淋巴细胞早期活化抗原CD69的表达的影响;利用噻唑蓝(MTT)比色法观察BisⅧ对JurkatT细胞增殖影响;使用倒置显微镜观察BisⅧ对Jurkat T细胞集落形成的情况的影响;采用碘化丙锭(PI)染色检测BisⅧ对Jurkat T细胞周期的影响;应用钙黄绿素(Calcien-AM)染色检测BisⅧ对JurkatT细胞凋亡的影响。结果终浓度为5、10、20μmol/L的BisⅧ对Jurkat T细胞的早期活化抗原CD69的表达具有明显的抑制作用(P<0.05);BisⅧ对Jurkat T细胞增殖具有显著的抑制作用(P<0.01),且呈剂量依赖性。随着BisⅧ浓度的增高,Jurkat T细胞停滞于G0/G1期;BisⅧ对JurkatT细胞凋亡具有明显的促进作用,并呈剂量依赖性。结论 BisⅧ对JurkatT细胞细胞生物学行为有抑制作用,是潜在治疗白血病的抗肿瘤药物。
Objective To study the effects of BisⅧ on the activation, proliferation, cycle and apoptosis of Jurkat T cells. The anti-tumor mechanism of BisⅧ was also discussed. Methods Jurkat T cells were used as a model. The effect of BisⅧ on the expression of CD69, an early activation antigen of T lymphocytes, was examined by dual-color fluorescent antibody staining combined with flow cytometry. The effect of BisⅧ on the proliferation of Jurkat T cells was observed by MTT assay The effects of BisⅧ on Jurkat T cell colony formation were observed by inverted microscopy. The effect of BisⅧ on the cycle of Jurkat T cells was detected by propidium iodide (PI) staining. The effect of BisⅧ on Jurkat T cells was detected by Calcium-AM staining. Effect of apoptosis. Results BisⅧ at final concentrations of 5, 10 and 20 μmol / L significantly inhibited the expression of CD69 on Jurkat T cells (P <0.05), while BisⅧ inhibited the proliferation of Jurkat T cells significantly (P <0.01) ), And in a dose-dependent manner. Jurkat T cells arrested in G0 / G1 phase with the increase of Bis ¢ öt concentration; Bis ¢ ñincreased significantly the apoptosis of Jurkat T cells in a dose-dependent manner. Conclusion BisⅧ inhibits the biological behavior of Jurkat T cells and is a potential anti-tumor agent for the treatment of leukemia.