论文部分内容阅读
目的和方法利用细菌培养、电镜观察和聚合酶链反应等方法对幽门螺杆菌（ＨｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉＨｐ）的二种形态（螺旋状体和圆球体）尤其是圆球体的细菌学、形态学和遗传学等进行研究。结果随着培养时间的延长，螺旋状体逐渐转换为圆球体。部分圆球体系退行性变；而一部分圆球体具有完整的细胞膜并有完好的鞭毛，这部分Ｈｐ圆球体可能具有活性。Ｈｐ螺旋状体和圆球体都分泌过氧化氢酶、碱性磷酸酶及酸性磷酸酶，ＰＣＲ法提示Ｈｐ圆球体仍然保留螺旋状体所具有的重要基因如尿素酶Ｃ基因及Ｈｐ种属特异性２６ｋＤ蛋白基因。结论本研究支持至少部分Ｈｐ圆球体可能具有活性但体外不能培养这一假说。
OBJECTIVE AND METHODS Bacteriology, morphology and genetics of Helicobacter pylori Hp in two forms (helical bodies and spheroids), especially spheroids, were performed by means of bacterial culture, electron microscopy and polymerase chain reaction the study. Results With the extension of culture time, the spiral body gradually transformed into a sphere. Part of the ball system degenerative changes; and some of the spheres have a complete cell membrane and have intact flagella, this part of the Hp spheroid may be active. Both Hp helices and spheroids secreted catalase, alkaline phosphatase and acid phosphatase. The results of PCR showed that Hp spheroids retained the specific genes of helix-like genes such as urease C and Hp 26kD protein gene. Conclusions This study supports the hypothesis that at least some Hp spheroids may be active but not cultured in vitro.