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叶色突变体作为一种易于观察的性状突变,是开展光合系统的结构和功能、叶绿素生物合成及其调控机制研究的理想材料。本研究以水稻T-DNA插入细条纹叶色突变体为研究材料,通过叶片的形态鉴定、石蜡切片、叶绿素和光合速率测定,分析突变体的表型和光合特性;采用TAIL-PCR技术,定位和克隆该突变基因。结果表明:突变体苗期幼叶上呈现间断的白色细条纹,而叶鞘、叶脉表现正常,三叶期后至分蘖期叶片逐渐转绿;突变体叶色对温度变化敏感;是国内外尚未报道的一种新型水稻细条纹叶色突变体。根据T-DNA插入标签,确定突变候选基因位于第7染色体上,并对候选基因进行了克隆已获得部分cDNA序列,暂命名为fs3(t)。
Leaf color mutant, as a kind of easily-observed trait mutation, is an ideal material for studying the structure and function of photosynthetic system, chlorophyll biosynthesis and its regulation mechanism. In this study, the rice stripe leaf mutant with T-DNA was inserted into the study material. The morphological and photosynthetic characteristics of the mutant were analyzed by morphological identification, paraffin section, chlorophyll and photosynthetic rate. TAIL-PCR And cloning the mutant gene. The results showed that the mutant showed intermittent white fine streaks on the young leaves, while the leaf sheaths and veins showed normal, while the leaves from the third leaf stage to the tillering stage turned green gradually. The leaf color of the mutant was not sensitive to temperature change at home and abroad A new type of rice stripe leaf color mutant. Based on the T-DNA insertion tag, the candidate gene was identified as being located on chromosome 7, and a partial cDNA sequence was obtained by cloning the candidate gene, tentatively named as fs3 (t).