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目的研究冷晶体及温血停搏液对心肌的保护作用。方法将96只猫随机分为体外循环(CPB)组(Ⅰ)、损伤组(Ⅱ)、冷晶保护组(Ⅲ)及温血保护组(Ⅳ)。观测各组心功能、膜ATP酶活性和膜蛋白颗粒的变化。结果组Ⅰ各时间点各项指标无变化。缺血期间,组ⅡⅣNaKATPase活性下降(P<0.01);组Ⅱ、ⅢCaMgATPase活性下降,膜蛋白颗粒数减少(P<0.05),组Ⅳ无减少(P>0.05)。复灌后,组Ⅳ心功能、CaMgATPase活性恢复正常,NaKATPase活性和膜蛋白颗粒数恢复稍差。结论单纯CPB对心功能、膜蛋白无损伤作用;温血停搏液心肌保护作用较好,但仍需完善。
Objective To study the protective effect of cold crystal and warm blood cardioplegia on myocardium. Methods 96 cats were randomly divided into CPB group (Ⅰ), injury group (Ⅱ), cold crystal protection group (Ⅲ) and warm - blooded protection group (Ⅳ). The changes of cardiac function, membrane ATPase activity and membrane protein granules in each group were observed. Results There was no change in each index in group Ⅰ at all time points. During ischemia, the activity of Group ⅡNaKATPase decreased (P <0.01); the activity of CaMgATPase in group Ⅱ and Ⅲ decreased and the number of membrane protein decreased (P <0.05) .05). After reperfusion, heart function of group Ⅳ, CaMgATPase activity returned to normal, NaKATPase activity and the number of membrane protein particles recovered slightly worse. Conclusions CPB alone has no effect on cardiac function and membrane protein. Cardioprotection by warm blood cardioplegia is better, but it still needs improvement.