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目的以响应面法优化梅花状青霉代谢抗氧化活性物质的培养条件。方法以目标菌株发酵液浸膏抗氧化活性为响应值,考察培养时间、碳源、氮源与浸膏抗氧化活性之间的相关性。以单因素实验结果为基础,利用Box-Behnken设计组合实验方案,采用Design-Expert 8.05软件优化菌株的实验室培养条件参数。结果菌株代谢抗氧化活性物质最优培养条件为:培养时间为16d,碳源为葡萄糖20.0g·L~(-1),氮源为蛋白胨10.8g·L~(-1),平行测试DPPH自由基清除率为97.83%,其IC50为13.57μg·mL~(-1)。结论响应曲面法回归模型的预测值与实测值接近,拟合较好,该方法可用于类似菌株活性物质获取条件的优化。
Objective To optimize the culture conditions of metabolites and anti-oxidative substances of Penicillium decumbens by response surface methodology. Methods The antioxidant activity of the fermentation broth of the target strain was used as a response value to investigate the correlation between culture time, carbon source and nitrogen source and the antioxidant activity of the extract. Based on the single-factor experiment results, the design protocol of Box-Behnken was used to optimize the laboratory culture conditions of the strains using Design-Expert 8.05 software. Results The optimal culture conditions for the metabolites were: culture time 16d, carbon source 20.0g · L -1, peptone 10.8g · L -1, parallel DPPH The basal clearance rate was 97.83% and its IC50 was 13.57μg · mL -1. Conclusion The predictive value of the response surface method regression model is close to the measured value, and the fitting result is good. The method can be used to optimize the conditions for obtaining the active substances of similar strains.