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目的:探讨内质网应激分子葡萄糖调节蛋白78(GRP78)对非小细胞肺癌(NSCLC)的诊断价值。方法:收集45例NSCLC患者术后癌组织标本,及其18例癌旁组织,采用实时定量PCR法检测所有组织中GRP78的表达情况,分析NSCLC癌组织中GRP78的表达与患者临床特征的关系,及其对患者生存期的影响。结果:NSCLC组织中GRP78高表达率明显高于癌旁组织,差异有统计学意义(P<0.05)。而Ⅲa期NSCLC患者癌组织中GRP78的表达水平明显高于Ⅰ-Ⅱ期,两者存在显著性的差异(P<0.05)。Kaplan-Meier分析显示,GRP78高表达的患者其生存期明显低于GRP78低表达的患者(P<0.05)。结论:NSCLC患者GRP78的表达可能与肿瘤细胞的发生、发展及患者的生存期有关,可作为一个预测NSCLC患者诊断及治疗的重要的分子标志物。
Objective: To investigate the diagnostic value of endoplasmic reticulum stress molecule glucose regulatory protein 78 (GRP78) in non-small cell lung cancer (NSCLC). Methods: Forty-five specimens of NSCLC were collected and their adjacent tissues were collected. Real-time quantitative PCR was used to detect the expression of GRP78 in all tissues. The relationship between the expression of GRP78 in NSCLC and the clinical features was analyzed. And its impact on patient survival. Results: The high expression rate of GRP78 in NSCLC tissues was significantly higher than that in paracancerous tissues (P <0.05). However, the expression of GRP78 in stage Ⅲa NSCLC patients was significantly higher than that in stage Ⅰ-Ⅱ, there was a significant difference (P <0.05). Kaplan-Meier analysis showed that patients with GRP78 overexpression had significantly lower survival than those with low GRP78 expression (P <0.05). Conclusion: The expression of GRP78 in patients with NSCLC may be related to the occurrence and development of tumor cells and the survival of patients. It may be used as an important molecular marker in the diagnosis and treatment of NSCLC.