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采用免疫标记技术(TES一IEST、AWA一IEST和TES一IFAT)及酶联免疫印渍技术(ELIB)对卫氏并殖吸虫,华支睾吸虫和姜片虫成虫可溶性抗原与日本血吸虫卵和成虫可溶性抗原之间的交叉反应性及其表位分子基础进行了研究。710份上述3种吸虫病人血清与日本血吸虫抗原之间的交叉反应率分别为3.9%(10/259),4.2%(11/261)和3.2%(6/190).用ELIB对上述吸虫组分抗原分析结果表明,卫氏并殖吸虫、华支睾吸虫和姜片虫抗原与日本血吸虫抗原之间的交叉反应表位分子量分别为97~76ku,40~14.5ku和106~14ku,华支睾吸虫和姜片虫成虫抗原之间显示67~14ku,范围的交又反应表位分子量。本研究为今后纯化抗原,提高血清学方法的特异性和敏感性,提供了重要依据。
The soluble antigen of Paragonimus westermani, Clonorchis sinensis and Zingiber officinale were compared with that of Schistosoma japonicum by using immunofluorescence labeling (TES-IEST, AWA-IEST and TES-IFAT) and ELIB The cross-reactivity between adult soluble antigens and the molecular basis of their epitopes were studied. The cross-reactivity rates of sera from 710 strains of the three species of sucking worms with those of S. japonicum were 3.9% (10/259), 4.2% (11/261) and 3.2% (6/190), respectively. ELIB analysis of the components of the flukes showed that the molecular weight of the cross-reactivity epitopes of Paragonimus westermani, Clonorchis sinensis and Zingiberis antigen with Schistosoma japonicum antigens were 97-76 ku, 40-14.5 ku And 106 ~ 14ku, between the clonorchis sinensis and zooxanthella adult antigen showed 67 ~ 14ku, the scope of the intersection of reaction and epitope molecular weight. This study provides an important basis for the future purification of antigens, enhance the specificity and sensitivity of serological methods.