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【目的】探讨钝顶螺旋藻多糖(PSP)抗单纯疱疹病毒2型(HSV-2)的作用及可能机制,为进一步开发该药提供理论依据。【方法】实验选用HSV-2标准株(333株),以不同剂量的PSP作用于HSV-2复制周期的不同阶段,终点以病毒半数感染量(TCID50)、细胞病变效应(CPE)、MTT染色细胞保护率(MTT法)及荧光定量PCR(FQ-PCR)作为评价手段,判断PSP的抗病毒效果。【结果】PSP对Vero细胞毒性极低(TC50为1750μg/mL),对HSV-2无直接灭活作用,可抑制病毒吸附和感染细胞内病毒的复制,其中50μg/mL、100μg/mL、200μg/mL浓度的PSP对病毒复制的抑制率分别为63.94%、81.41%、85.63%;FQ-PCR结果显示随着PSP浓度及作用时间的增加,PSP对HSV-2DNA的抑制作用明显增强,具有良好的剂量和时效关系。【结论】PSP在体外具显著的抗HSV-2病毒作用,此作用发生在病毒吸附、病毒基因复制等多个环节上。
【Objective】 The purpose of this study was to investigate the role of PSP against HSV-2 and to provide a theoretical basis for further development of this drug. 【Methods】 The HSV-2 strain (333 strains) was selected in this study. Different doses of PSP were used to treat HSV-2 at different stages of replication. The endpoints were infected with TCID50, CPE and MTT Cell protection rate (MTT method) and fluorescence quantitative PCR (FQ-PCR) as an evaluation method to determine the PSP antiviral effect. 【Results】 PSP showed no cytotoxic effect on Vero cells (TC50 was 1750μg / mL) and no direct inactivation of HSV-2 inhibited the virus replication and replication in infected cells. 50μg / mL, 100μg / mL and 200μg / mL concentration of PSP on viral replication were 63.94%, 81.41%, 85.63% respectively; FQ-PCR results showed that the inhibitory effect of PSP on HSV-2 DNA was significantly enhanced with the increase of PSP concentration and time, The dose and aging relationship. 【Conclusion】 PSP has a significant anti-HSV-2 effect in vitro. This effect occurs in many aspects such as virus adsorption and virus gene duplication.