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目的:观察宽叶缬草对血管性痴呆模型小鼠学习记忆及海马区神经元病理学改变的影响。方法:采用反复夹闭双侧颈总动脉结合腹腔注射硝普钠的方法复制小鼠拟血管性痴呆的模型。健康昆明小鼠54只,随机分为3组:假手术组、血管性痴呆模型组、宽叶缬草组。分别于术后7、15、30 d,跳台实验检测其痴呆程度;透射电镜、HE和Nissl染色对海马区神经元病理学改变进行观察。结果:宽叶缬草组跳台实验潜伏期明显短于模型组(P<0.01),受电击总时间少于模型组(P<0.01);宽叶缬草组海马CA1区神经元数量明显多于模型组(P<0.01);模型组可见神经元脱失、部分神经细胞核固缩并有胶质细胞增生等病理学改变。结论:宽叶缬草能明显减轻血管性痴呆小鼠海马CA1区神经元损伤,改善脑缺血引起的学习记忆障碍。
Objective: To observe the effects of Valeriana officinalis on learning and memory and hippocampal neuronal pathological changes in vascular dementia model mice. METHODS: The model of vascular dementia in mice was replicated by repeatedly clipping the bilateral common carotid artery combined with intraperitoneal injection of sodium nitroprusside. Fifty-four healthy Kunming mice were randomly divided into three groups: sham operation group, vascular dementia model group, and broadleaf weed grass group. At 7th, 15th, and 30th day after operation, the degree of dementia was detected by a step-down test. Transmission electron microscope, HE, and Nissl staining were used to observe the neuronal pathological changes in the hippocampus. RESULTS: The incubation period of the stepping test in the broadleaved weeds group was significantly shorter than that of the model group (P<0.01), and the total time of the shock was less than that of the model group (P<0.01). The number of neurons in the CA1 area of the hippocampus was more than that of the model. Group (P <0.01); model group showed neuronal loss, some nerve cell nuclear pyknosis and glial cell proliferation and other pathological changes. Conclusion: Valeriana can significantly reduce neuronal damage in hippocampal CA1 region of vascular dementia mice and improve learning and memory impairment caused by cerebral ischemia.