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目的:研究莪术多糖对猪血清所致肝纤维化大鼠的保护作用。方法:大鼠50只腹腔注射异源猪血清(0.4ml/kg,每周3次,持续8周)复制肝纤维化动物模型。随机分成3组:模型对照组、莪术多糖治疗组(200,400mg/kg),持续灌胃30 d,同时平行设置正常大鼠10只作为正常对照组,对照组均ig给予等体积的生理盐水。末次给药后12h,检测血清丙氨酸转氨酶(ALT),谷草转氨酶(AST)和碱性磷酸酶(ALP)的水平以及测定肝匀浆透明质酸(HA),Ⅲ型前胶原(PCIII),层粘连蛋白(LN)的含量。HE染色观察肝组织组织病理学变化;Western blotting法检测肝组织α-平滑肌动蛋白(α-SMA)、转化生长因子β1(TGFβ1)的表达。结果:与模型对照组比较,莪术多糖(200,400mg/kg)以剂量依赖性地降低猪血清所致大鼠肝纤维化血清肝功能酶水平,同时减少肝组织中胶原蛋白的含量,并显著下调内源性α-SMA和TGFβ1的表达水平,从而缓解肝纤维化病情。结论:莪术多糖具有明显的抗肝纤维化作用,其机制可能与抑制肝纤维化大鼠胶原蛋白过表达以及TGF信号通路有关。
Objective: To study the protective effect of Curcuma phaeocaulis polysaccharides on pig serum induced liver fibrosis in rats. Methods: Allogeneic pig serum (0.4ml / kg, 3 times a week for 8 weeks) was injected intraperitoneally into 50 rats. The rats were randomly divided into three groups: model control group and Curcuma polysaccharide treatment group (200, 400mg / kg). The rats were continuously gavaged for 30 days. Ten normal rats were set as normal control group. The control group were given equal volume of normal saline. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) and serum levels of HA, PCIII, , Laminin (LN) content. The histopathological changes of liver tissue were observed by HE staining. The expression of α-smooth muscle actin (α-SMA) and transforming growth factor-β1 (TGFβ1) in liver tissue were detected by Western blotting. Results: Compared with the model control group, Curcuma polysaccharide (200, 400 mg / kg) reduced the serum hepatic function enzyme activity of liver fibrosis induced by pig serum in a dose-dependent manner and decreased the content of collagen in liver tissue significantly Endogenous α-SMA and TGFβ1 expression levels, thereby alleviating liver fibrosis. Conclusion: Curcuma polysaccharides has obvious anti-hepatic fibrosis effect, and its mechanism may be related to the inhibition of collagen overexpression and TGF signaling pathway in hepatic fibrosis rats.