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目的研究抑癌基因KLF-6和APC在结直肠癌中的表达规律及其临床意义。方法应用RT-PCR和免疫组织化学法对32例结直肠癌组织和正常黏膜组织中KLF-6和APC基因mRNA及蛋白的表达进行检测和分析。结果KLF-6与APC的mRNA阳性表达率在结直肠癌组织中分别为37．5%和34．3%,明显低于正常结直肠黏膜组织的96．9%和93．8%(P＜0．05)。KLF-6与APC的免疫组化染色阳性表达率在结直肠癌组织中分别为28．1%和25．0%,明显低于正常结直肠黏膜组织的81．3%和84．4%(P＜0．05)。KLF-6与APC在结直肠癌中的表达存在相关性(P＜0．05),并与肿瘤组织的分化程度、浸润深度、淋巴结转移及临床分期均有明显相关性(P＜0．05)。结论抑癌基因KLF-6和APC的低表达可能与结直肠癌的发生、发展、转移及预后有关,且两者存在相关性。
Objective To study the expression and clinical significance of tumor suppressor gene KLF-6 and APC in colorectal cancer. Methods The mRNA and protein expressions of KLF-6 and APC in 32 colorectal cancer tissues and normal mucosa tissues were detected by RT-PCR and immunohistochemistry. Results The mRNA expression of KLF-6 and APC was 37.5% and 34.3% in colorectal cancer tissues, which was significantly lower than that in normal colorectal mucosa (96.9% and 93.8%, respectively) (P < 0.05). The positive rate of immunohistochemical staining of KLF-6 and APC was 28.1% and 25.0% in colorectal cancer tissues, which was significantly lower than that of normal colorectal mucosa (81.3% vs 84.4%, respectively) P <0.05). There was a significant correlation between KLF-6 and APC expression in colorectal cancer (P <0.05), and there was a significant correlation between the expression of KLF-6 and tumor differentiation, depth of invasion, lymph node metastasis and clinical stage (P <0.05 ). Conclusion The low expression of tumor suppressor genes KLF-6 and APC may be related to the occurrence, development, metastasis and prognosis of colorectal cancer, and there is a correlation between them.