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为了初步探索猪的李斯特菌携带情况以及屠宰加工环境中污染的可能性,并进一步对分离到的单增李斯特菌进行分型分析,以了解其分子流行病学特征,为预防控制食源性李斯特菌病提供参考。2011年3月至2012年2月每月月初定点采集某一屠宰场的猪粪便标本和加工场所环境水样标本,进行李斯特菌的病原学分离,对分离到的单增李斯特菌进行血清分型、多位点序列分型(MLST)和脉冲场电泳分型(PFGE)分析。1322份猪粪便标本和104份环境标本中,共分离到7株单增李斯特菌、56株无害李斯特菌。5株单增李斯特菌属于1/2c血清型(ST9型),1株1/2a血清型(ST199型),1株1/2b血清型(ST5型)。4株1/2c型菌株具有完全相同的PFEG带型,与另外1株1/2c型菌株具有相似带型,而与其他2株菌株(分别为1/2a型和1/2b型)的带型具有较大差异。猪的单增李斯特菌携带率较低,但存在污染加工环境的可能性,进而导致生猪肉食品受到污染。对屠宰及加工场所采取有效的消毒灭菌措施能够避免单增李斯特菌的污染和传播。
In order to explore the possibility of carrying Listeria pig in pigs and the possibility of contamination in slaughter and processing environment and further analyze the typing of isolated Listeria monocytogenes to understand its molecular epidemiological characteristics and to prevent and control food sources Listeria monocytogenes provide a reference. From March 2011 to February 2012, monthly pig stool samples and environmental samples were collected from a slaughterhouse site for pathogenic isolation of Listeria monocytogenes from March 2011 to February 2012 respectively. Serum Typing, multilocus sequence typing (MLST) and pulse field electrophoresis typing (PFGE) analysis. Of 1322 swine stool samples and 104 environmental samples, seven Listeria monocytogenes and 56 Listeria monocytogenes were isolated. Five Listeria monocytogenes belonged to serogroups 1 / 2c (ST9), 1 serotype 1 / 2a (ST199) and 1 serotype 1 / 2b (ST5). Four isolates of type 1 / 2c had the same PFEG band pattern, which was similar to the other 1 / 2c strains, but the bands of the other two isolates (type 1 / 2a and 1 / 2b) Type has a big difference. Pig Listeria monocytogenes carrying rate is lower, but there is the possibility of contamination of the processing environment, resulting in raw pork contaminated food. Effective disinfection and sterilization of slaughtering and processing sites can avoid the contamination and spread of Listeria monocytogenes.