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目的了解除草剂特拉津是否具有诱变性。方法采用小鼠淋巴瘤细胞TK基因突变试验,设6.25、12.5、25、50、100μg/ml5个剂量组,不加S9条件下处理L5178Y细胞3h和12h,加S9条件下处理细胞3h,分别进行细胞毒性和突变频率的测定。结果随阿特拉津染毒剂量的增加,3种处理条件下各剂量组的相对存活率(RV)和相对悬浮增长率(RSG)均降低,突变频率增高,且有剂量反应关系;在100μg/ml剂量组L5178Y细胞tk位点突变频率与溶剂对照组自发突变频率相比差异有统计学意义。结论阿特拉津对L5178Y细胞有明显的细胞毒性和一定的遗传毒性。
Aim To understand whether herbicide trazin is mutagenic. Methods The TK gene mutation of mouse lymphoma cells was used to test the effect of TK gene mutation in mice at 5, 6, 25, 25, 50 and 100μg / ml for 5h, 5h and 12h respectively. The cells were treated with S9 for 3h, Determination of cytotoxicity and mutation frequency. Results With the increase of atrazine dose, the relative survival rate (RV) and relative suspension growth rate (RSG) of all the three treatment groups decreased, the mutation frequency increased, and dose-response relationship; / ml dose L5178Y cell tk mutation frequency and solvent control group spontaneous mutation frequency difference was statistically significant. Conclusion Atrazine has obvious cytotoxicity and certain genotoxicity on L5178Y cells.