论文部分内容阅读
目的探讨重度子痫前期病人血清体液免疫状况与其外周血胎儿有核红细胞(FNRBC)水平的关系。方法随机选择重度子痫前期孕妇33例(研究组)及同期正常孕妇20例(对照组),采用免疫比浊法和Greiss反应法测定血清中的免疫球蛋白(IgG、IgMI、gA)与补体C3、C4水平,采用单密度梯度离心法富集计算FNRBC数量。结果研究组孕妇外周血中FNRBC数量较对照组明显升高(t=10.78,P<0.05);研究组体液免疫指标中IgG、IgM及C3水平明显低于对照组(t=5.36~9.10,P<0.05)。研究组FNRBC数量与IgGI、gM及C3水平呈负相关(r=-0.89~-0.44,P<0.05)。结论重度子痫前期孕妇外周血中FNRBC数量增多与机体体液免疫状况密切相关,胎儿物质向母血的渗漏增多、机体免疫调控失衡和紊乱均可能是重度子痫前期发生发展的重要因素。
Objective To investigate the relationship between the serum humoral immunity and the level of fetal nucleated erythrocytes (FNRBC) in patients with severe preeclampsia. Methods Thirty-three pregnant women with severe preeclampsia (study group) and 20 normal pregnant women (control group) were enrolled in this study. Serum immunoglobulin (IgG, IgMI, gA) and complement were detected by immunoturbidimetry and Greiss reaction C3 and C4 levels, and the number of FNRBCs was calculated by single density gradient centrifugation. Results The number of FNRBC in the peripheral blood of the study group was significantly higher than that of the control group (t = 10.78, P <0.05). The levels of IgG, IgM and C3 in the humoral immunity of the study group were significantly lower than those of the control group (t = 5.36-9.10, P <0.05). The number of FNRBC in the study group was negatively correlated with the levels of IgGI, gM and C3 (r = -0.89-0.44, P <0.05). Conclusions The increase of peripheral blood FNRBC in severe preeclampsia is closely related to the body fluid immune status. The leakage of fetal substance to maternal blood is increased. The imbalance and disorder of immune regulation may be important factors in the development of severe preeclampsia.