建立了利用反相高效液相色谱法同时测定保健口服液中２０种甾体激素的方法，进行了色谱条件的优化和样品预处理方法的探讨，并应用于市售保健口服液中微量激素的测定。最低检出量为０．１６～３．１８ｎｇ，回收率为８０．５％～１０３．１％，相对标准偏差为３．４％～９．２％。 A method for the simultaneous determination of 20 steroid hormones in health oral liquid by reversed-phase high performance liquid chromatography was established. The optimization of chromatographic conditions and the method of sample pretreatment were also discussed. The method was applied to the determination of trace hormones Determination. The lowest detection limit was 0.16 ~ 3.18ng, the recovery was 80.5% ~ 103.1%, the relative standard deviation was 3.4% ~ 9.2%.