A detailed understanding of genetic architecture of mRNA expression by millions of genetic variants is important for studying quantitative trait variation.In this study,we identified 1.25M SNPs with a minor allele frequency greater than 0.05 by combining reduced genome sequencing (GBS),highdensity array technologies (600K),and previous deep RNA-sequencing data from 368 diverse inbred lines of maize.The balanced allelic frequencies and distributions in a relatively large and diverse natural panel helped to identify expression quantitative trait loci (eQTLs) associated with more than 18 000 genes (63.4％ of tested genes).We found that distant eQTLs were more frequent (～75％ of all eQTLs)across the whole genome.Thirteen novel associated loci affecting maize keel oil concentration were identified using the new dataset,among which one intergenic locus affected the keel oil variation by controlling expression of three other known oil-related genes.Altogether,this study provides resources for expanding our understanding of cellular regulatory mechanisms of transcriptome variation and the landscape of functional variants within the maize genome,thereby enhancing the understanding of quantitative variations.