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作者于1987年对居住在巴西东北部巴拉州和马托格罗索州的4个印第安部族进行寄生虫学与血清学调查,以确定那些住在丛林深处孤立且较原始的部族的疟疾是否以猴作为其贮存宿主。从390人中各抽取静脉血5ml,分离血清保存于液氮,回实验室后置于-70℃待检,厚薄血膜用吉氏液染色并镜检。感染了巴西疟原虫或体外培养的恶性疟原虫的费氏按蚊经解剖后获得子孢子,并从巴拉巴按蚊感染泰国间日疟病人获得子孢子作抗原;从感染的恶性疟或间日疟和巴西疟原虫的红细胞作抗
The authors conducted a parasite and serological survey of four Indian tribes living in Barra and Mato Grosso states in northeastern Brazil in 1987 to identify those malaria that lived in isolated and pristine tribes deep in the jungle Whether the monkey as its storage host. Blood samples were taken from 390 blood samples in 5 ml, and the sera were separated and stored in liquid nitrogen. After returning to the laboratory, the samples were stored at -70 ° C. Thick and thick blood films were stained with Giemsa and examined microscopically. Anopheles festivae infected with Plasmodium falciparum or P. falciparum in vitro were dissected to obtain sporozoites and sporozoites were obtained from Anopheles stephensi infected with P. thaliana in the Arabidopsis thaliana as antigen. Malaria and red blood cells of Plasmodium falciparum for anti-malaria