目的　克隆和分析与肝细胞癌发生发展相关的基因。方法　应用差异显示技术获得2 4例肝癌及其癌旁组织表达差异的目标基因片段 ,对其进行克隆、测序 ,并在基因数据库中检索进行同源性比较 ,应用印迹法 (Northern)杂交技术研究其正常组织分布和肝癌中表达的变化。结果　肝癌组织中获得一目标基因片段经测序证明其来源于一已知基因核糖体蛋白L2 6(RPL2 6) ,North ern杂交分析表明该基因在各种组织中广泛表达 ,83 .3 %原发性肝癌患者癌组织中RPL2 6表达显著高于癌旁组织。结论　RLP2 6可能是肝癌进展的有用的生物学标志 Objective To clone and analyze genes involved in the development of hepatocellular carcinoma. Methods Differential display technology was used to obtain target gene fragments of 24 cases of hepatocellular carcinoma and paracancerous tissues. The target genes were cloned, sequenced, and searched in the gene database for homology comparison. Northern blot was used for the study. Its normal tissue distribution and expression changes in liver cancer. RESULTS: A target gene fragment obtained from hepatocellular carcinoma was sequenced to prove that it was derived from a known gene ribosomal protein L2 6 (RPL26). Northern blot analysis showed that the gene was widely expressed in various tissues with a peak of 83. 3%. The expression of RPL2 6 was significantly higher in cancer tissue than that in adjacent tissues. Conclusion RLP2 6 may be a useful biological marker for liver cancer progression